Purified anti-EGFR Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 101	1

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human EGFR surrounding Tyrosine 869 (Tyrosine 845 if signal peptide is excluded)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
ICC, IP, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended.? For immunoprecipitation, the suggested use of this reagent is 2.5 ?g per ml.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using PFA-fixed A431 cells permabilized with methanol or Triton X-100. While both permeabilization methods were compatible with the antibody, Triton X-100 enabled superior staining.

This clone is predicted to recognize only isoforms I and V of EGFR due to the absence of the immunizing epitope in the other isoforms.
RRID: AB_2810671 (BioLegend Cat. No. 617501) AB_2810672 (BioLegend Cat. No. 617502)