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Go-ChIP-Grade™ Purified anti-STAT1 Phospho (
产品名称:
Go-ChIP-Grade™ Purified anti-STAT1 Phospho (
产品类别:
抗体
产品编号:
666405
产品应用:
666405
[价格]
规格 价格 库存
25µg ¥ 105 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICFC, ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 by volume.?For Western blotting, the suggested use of this reagent is 0.1 - 2.0 ?g per ml.?For?intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume.?For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. It is recommended that the reagent to be titrated for optimal performance before each experiment.

For intracellular flow cytometry our True-Nuclear? Transcription Factor Staining Protocol is recommended.

Application Notes

Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701).

When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.

RRID
AB_2728501 (BioLegend Cat. No. 666405) AB_2728502 (BioLegend Cat. No. 666406)

Antigen Details

Structure
Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Distribution

Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution

Function
Immune response activation, cell signaling
Interaction
STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
Biology Area
Cell Biology
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Moretti S, et al. 2017. J. Biol. Chem. 292: 1785.
  2. Wei J, et al. 2015. J. Immunol. 195: 2870.
  3. Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443
  4. Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909.
  5. Wu TR, et al. 2002. J. Biol. Chem. 277: 47572.
  6. Horvath, et al. 1996. J. Virol. 70: 647.
  7. Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 Phospho Tyr701 on UniProt.org

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