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Purified anti-BTK Phospho (Tyr223)/ITK Phospho (Ty
产品名称:
Purified anti-BTK Phospho (Tyr223)/ITK Phospho (Ty
产品类别:
抗体
产品编号:
605201
产品应用:
605201
[价格]
规格 价格 库存
25µg ¥ 112 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BTK peptide phosphorylated at Tyr 223, Complete Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB -?Quality tested
ICFC -?Verified?

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is?2.0 ug/mL (1:250 dilution).?For?intracellular flow cytometric staining, the suggested use of this reagent is 0.25 ?g per million cells in 100 ?l volume.?It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clones A16128C and A16128B (anti-BTK Phospho (Tyr223)) both bind BTK Phospho Tyr223 with similar affinity, but in comparison to clone A16128B, clone A16128C has a much stronger recognition of phosphorylated Tyrosine 180 of ITK by intracellular staining and flow cytometric analysis. ITK is the T cell-specific homolog of BTK that is not found in B cells. Similarly, T cells lack BTK1.?

Application References

(PubMed link indicates BioLegend citation)
  1. Tomlinson MG, et al. 2004. Mol. Cell. Biol. 24: 2455
RRID
AB_2721726 (BioLegend Cat. No. 605201) AB_2721727 (BioLegend Cat. No. 605202)

Antigen Details

Structure
BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).
Distribution

Cytosol, plasma-membrane associated, B cells

Function
Links B cell receptor engagement with downstream signaling events
Interaction
ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5
Ligand/Receptor
PIP3
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References

1. Krysiak K,?et al. 2017.?Blood.?129:473.
2. Burger JA,?et al. 2016.?Nat Commun. 7:11589.
3. Wang Q,?et al. 2015.?Elife. 4:e06074.
4.?Ito M,?et al. 2015.?Nat. Commun. 6:7360.
5.?Pillinger G,?et al. 2015.?Sci. Rep. 5:12949.
6. Cinar M,?et al. 2013.?Leuk. Res. 37:1271.
7. Doyle SL,?et al. 2007?J. Biol. Chem. 282:36953.
8. Nore BF,?et al. 2003.?Biochem. Biophys. Acta. 1645:123.
9.?Li Z,?et al. 1997.?Proc. Natl. Acad. Sci. 94:13820.

Gene ID
695 View all products for this Gene ID
UniProt
View information about BTK Phospho Tyr223/ITK Phospho Tyr180 on UniProt.org

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