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PE anti-BTK Phospho (Tyr223)/ITK Phospho (Tyr180)
产品名称：
PE anti-BTK Phospho (Tyr223)/ITK Phospho (Tyr180)
产品类别：
抗体
产品编号：
605203
产品应用：
605203
[价格]
规格 价格 库存
25tests ￥ 159 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BTK peptide phosphorylated at Tyr 223, Complete Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC -?Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clones A16128C and A16128B (anti-BTK Phospho (Tyr223)) both bind BTK Phospho Tyr223 with similar affinity, but in comparison to clone A16128B, clone A16128C has a much stronger recognition of phosphorylated Tyrosine 180 of ITK by intracellular staining and flow cytometric analysis. ITK is the T cell-specific homolog of BTK that is not found in B cells. Similarly, T cells lack BTK1.?

Application References

(PubMed link indicates BioLegend citation)
  1. Tomlinson MG, et al. 2004. Mol. Cell. Biol. 24: 2455
RRID
AB_2721622 (BioLegend Cat. No. 605203) AB_2721623 (BioLegend Cat. No. 605204)

Antigen Details

Structure
BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).
Distribution

Cytosol, plasma-membrane associated, B cells

Function
Links B cell receptor engagement with downstream signaling events
Interaction
ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5
Ligand/Receptor
PIP3
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References

1. Krysiak K,?et al. 2017.?Blood.?129:473.
2. Burger JA,?et al. 2016.?Nat Commun. 7:11589.
3. Wang Q,?et al. 2015.?Elife. 4:e06074.
4.?Ito M,?et al. 2015.?Nat. Commun. 6:7360.
5.?Pillinger G,?et al. 2015.?Sci. Rep. 5:12949.
6. Cinar M,?et al. 2013.?Leuk. Res. 37:1271.
7. Doyle SL,?et al. 2007?J. Biol. Chem. 282:36953.
8. Nore BF,?et al. 2003.?Biochem. Biophys. Acta. 1645:123.
9.?Li Z,?et al. 1997.?Proc. Natl. Acad. Sci. 94:13820.

Gene ID
695 View all products for this Gene ID
UniProt
View information about BTK Phospho Tyr223/ITK Phospho Tyr180 on UniProt.org

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