Purified anti-STAT3 Phospho (Ser727) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 112	1

规格

价格

库存

25µg

￥ 112

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic human STAT3 peptide phosphorylated at serine 727
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0?- 2.0??g/mL.?For immunocytochemistry, a concentration range of 1.0?- 5.0?μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clones A16089A and A16089B are both specific for STAT3 phosphorylated at serine 727, but performed differently for different applications tested:

Both clones displayed strong specificity and selectivity for WB, but clone A16089A displayed a considerably stronger affinity for the target compared to A16089B

Both clones performed well for ICC, though clone A16089A displayed mild extranuclear staining

A16089B performed well for ChIP, A16089A failed ChIP application testing
RRID: AB_2892483 (BioLegend Cat. No. 669501) AB_2892483 (BioLegend Cat. No. 669502)

STAT3 is a 770 amino acid protein with a predicted molecular weight of 88 kDa. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.

Distribution

Cytosol and nucleus/Ubiquitously expressed

Function

Transcription factor

Antigen References