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Purified anti-ZAP70 Phospho (Tyr292) Antibody
产品名称:
Purified anti-ZAP70 Phospho (Tyr292) Antibody
产品类别:
抗体
产品编号:
691902
产品应用:
691902
[价格]
规格 价格 库存
100µg ¥ 288 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human ZAP70 peptide phosphorylated at Y292.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 ?g per ml. Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Voisinne G, et al. 2022. Nat Immunol. 23:1355. PubMed
RRID
AB_2632759 (BioLegend Cat. No. 691902)

Antigen Details

Structure
619 amino acids with a predicted molecular weight of 70 kD.
Distribution

Cytoplasm and cell membrane.

Function
Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-γ1-dependent and Ras-dependent signaling cascades. Associates with PLC-γ 1.
Interaction
NFAM1, Cbl, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, CD247/CD3Z.
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9166.
2. Arpaia E, et al. 1994. Cell. 76:947.
3. Chan AC, et al. 1994. Science 264:1599.
4. Negishi I, et al. 1995. Nature 376:435.
5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279.
6. Klammt C, et al. 2015. Nat. Immunol. 16:961.
7. Weiss A, et al. 1993. Cell. 73:209.
8. Gong Q, et al. 2001. J. Exp. Med. 194:507.
9. Magnan A, et al. 2001. J. Exp. Med. 194:491.

Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP-70 Phospho Tyr292 on UniProt.org

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