Purified anti-STAT1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 101	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human STAT1 peptide phosphorylated at Serine 727.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.25 ?g per ml (1:2000 - 1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for STAT1 isoform a and is not predicted to recognize the ? isoform due to the absence of the region corresponding to the immunizing peptide.

This clone displayed a stronger affinity for STAT1a in parallel western blot testing with BioLegend’s anti-STAT1 antibody (clone 10C4B40).

U3A cells were selected for antibody specificity testing because the cell line does not express STAT1¹.

This clone was derived from a human STAT1 peptide phosphorylated at Serine 727 immunogen, but tested negative for phospho-recognition while displaying strong pan-reactivity. Supplemental data is provided confirming the differences in phosphorylation of STAT1 Serine 727 in HeLa cells treated with or without nocodazole.

Application References (PubMed link indicates BioLegend citation)

Muller M, et al. 1993. EMBO J. 12:4221

Product Citations

Kiritsy MC, et al. 2021. Elife. 10:. PubMed

RRID

AB_2749867 (BioLegend Cat. No. 603701) AB_2749868 (BioLegend Cat. No. 603702)

Antigen Details

Structure

STAT1α is a 750 amino acid protein with a predicted molecular weight of 87 kD; contains an SH2 domain responsible for homodimerization or heterodimerization.

Distribution

Ubiquitously expressed; cytosolic and nuclear distribution

Function

Transcription activator/Cytokine Signaling

Interaction

Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.

Biology Area

Cell Biology, Signal Transduction, Transcription Factors

Molecular Family

Nuclear Markers

Antigen References