Purified anti-LC3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 112	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

A synthetic peptide corresponding to N-terminal region of human LC3B protein (between amino acid residues 1-50) conjugated to KLH.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-P - Quality tested
ICC - Verified?
WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 ?g/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 1.0 ?g/mL is suggested ?It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with LC3A and LC3B isoforms.

Additional reported applications (for the relevant formats) include: western blotting

Product Citations

Jiang W, et al. 2022. Front Physiol. 13:949737. PubMed
Al–Ramahi I, et al. 2018. Cell Syst. 7:28. PubMed
Stykel MG, et al. 2021. Cell Reports. 35(6):109099. PubMed

RRID

AB_2650713 (BioLegend Cat. No. 848801) AB_2650714 (BioLegend Cat. No. 848802)

Antigen Details

Structure: LC3 is a 125 amino acid protein with a molecular mass of 14.7 kD.
Distribution: Tissue distribution: brain, heart, skeletal muscle, blood and testis.
Cellular distribution: cytosol, mitochondria, cytoskeleton, endoplasmic reticulum, cytoplasmic vesicles, autophagosome.
Function: LC3 proteins play a key role in the selective recruitment of autophagic cargoes into autophagosomes, and serve as docking sites for adaptor proteins.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family: Autophagosome Markers
Antigen References: 1. Lee J, et al. 2012. Biochem. J. 441(2):523. PubMed
2. Martini-Stoica H, et al. 2016. Trends Neurosci. 39(4)221. PubMed
Gene ID: 81631 View all products for this Gene ID
UniProt: View information about LC3 on UniProt.org

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