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Brilliant Violet 650™ anti-human CD127 (IL-7
产品名称：
Brilliant Violet 650™ anti-human CD127 (IL-7
产品类别：
抗体
产品编号：
351325
产品应用：
351325
[价格]
规格 价格 库存
25tests ￥ 226 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Application References

(PubMed link indicates BioLegend citation)
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  2. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  3. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  4. Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
  5. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  6. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  7. You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
  8. Wang J, et al. 2020. Cancer Immunol Res. 794:8. PubMed
  9. Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed
  10. Lo Tartaro D, et al. 2022. Cells. 11:. PubMed
  11. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  12. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  13. Hill DL, et al. 2019. J Exp Med. 216:1857. PubMed
  14. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
RRID
AB_11125369 (BioLegend Cat. No. 351325) AB_2562095 (BioLegend Cat. No. 351326)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

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