Alexa Fluor® 647 anti-TCF1 (TCF7) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 159	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Partial TCF1 recombinant protein (116-334 aa)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol.?For flow cytometric staining, the suggested use of this reagent is 5 ?l per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations

Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
Sung BY, et al. 2022. J Clin Invest. 132:. PubMed
Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed

RRID

AB_2566619 (BioLegend Cat. No. 655203) AB_2566620 (BioLegend Cat. No. 655204)

Antigen Details

Structure: 384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
Distribution: Nucleus
Function: TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Interaction: TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers, TCRs
Antigen References: 1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207.
2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060.
3. Wang R, et al. 2011. J. Immunol. 187:5964.
4. Weber BN, et al. 2011. Nature 476:63.
5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777.
6. Staal FJ, et al. 1999. Int. Immunol. 11:317.
Gene ID: 6932 View all products for this Gene ID
UniProt: View information about TCF1 on UniProt.org

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