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Purified anti-SPI1 (PU.1) Antibody
产品名称:
Purified anti-SPI1 (PU.1) Antibody
产品类别:
抗体
产品编号:
658002
产品应用:
658002
[价格]
规格 价格 库存
100µg ¥ 270 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SPI1 recombinant protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per ml.?For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations
  1. Yang L, et al. 2020. Cell Stem Cell. 27(1):125-136. PubMed
  2. Guttikonda SR, et al. 2021. Nat Neurosci. 24:343. PubMed
  3. Panagiotakopoulou V, et al. 2020. Nat Commun. 4.04375. PubMed
RRID
AB_2562720 (BioLegend Cat. No. 658002)

Antigen Details

Structure
270 amino acids, predicted molecular weight of 31 kD; contains a C-termianl ETS domain responsible for DNA binding.
Distribution

Nucleus.

Function
SPI1 is a transcription factor required for the development of lymphoid and myeloid cells.
Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmelé P, et al. 2010. Cancer Res. 70:6757.

Gene ID
6688 View all products for this Gene ID
UniProt
View information about SPI1 on UniProt.org

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