PE anti-SPI1 (PU.1) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 146	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Full length SPI1 recombinant protein expressed in E. coli.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.125??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations

Zhu H, et al. 2018. Elife. 7:. PubMed
Nott A, et al. 2021. Nature Protocols. 16(3):1629-1646. PubMed

RRID

AB_2629617 (BioLegend Cat. No. 681307) AB_2629618 (BioLegend Cat. No. 681308)

Antigen Details

Structure: 272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.
Distribution: Nucleus.
Function: SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.
Interaction: SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmele P, et al. 2010. Cancer Res. 70:6757.
Gene ID: 20375 View all products for this Gene ID
UniProt: View information about SPI1 on UniProt.org

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