Alexa Fluor® 488 anti-SPI1 (PU.1) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 159	1

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length SPI1 recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
RRID: AB_2629611 (BioLegend Cat. No. 681305) AB_2629612 (BioLegend Cat. No. 681306)

Antigen Details

Structure: 272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.
Distribution: Nucleus.
Function: SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.
Interaction: SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmele P, et al. 2010. Cancer Res. 70:6757.
Gene ID: 20375 View all products for this Gene ID
UniProt: View information about SPI1 on UniProt.org

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