Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antib-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 112	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB -?Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1: 5000 diluted (0.1-1.0 ?g/ml). For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes.

Product Citations

Recabal A, et al. 2020. Journal of Neurochemistry. 156(2):182-199. PubMed

RRID

AB_2721734 (BioLegend Cat. No. 369501) AB_2721735 (BioLegend Cat. No. 369502)

Antigen Details

Structure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution: Cytoplasm and the nucleus.
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.
Interaction: ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: 1. Futran AS,?et al.?2013.?Curr. Biol.?23:R972.?
2. Mendoza MC,?et al.?2011.?Trends Biochem. Sci.?36:320.?
3. Chambard JC,?et al.?2007.?Biochim. Biophys. Acta.?1773:1299.?
4. Roux PP,?et al.?2004.?Microbiol. Mol. Biol. Rev.?68:320.?
5. Torii S,?et al.?2004.?J. Biochem.?136:557.?
6. Clark EA,?et al.?1996.?J. Biol. Chem.?271:14814.
Gene ID: 5594 View all products for this Gene ID
UniProt: View information about ERK1/2 Phospho Thr202 Tyr204 on UniProt.org

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