Brilliant Violet 421™ anti-ERK1/2 Phospho (T-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 182	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes.

Product Citations

Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed

RRID

AB_2629708 (BioLegend Cat. No. 369509) AB_2629709 (BioLegend Cat. No. 369510)

Antigen Details

Structure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution: Cytoplasm and the nucleus.
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.
Interaction: ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Phospho-Proteins, Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References: 1. Futran AS,?et al.?2013.?Curr. Biol.?23:R972.?
2. Mendoza MC,?et al.?2011.?Trends Biochem. Sci.?36:320.?
3. Chambard JC,?et al.?2007.?Biochim. Biophys. Acta.?1773:1299.?
4. Roux PP,?et al.?2004.?Microbiol. Mol. Biol. Rev.?68:320.?
5. Torii S,?et al.?2004.?J. Biochem.?136:557.?
6. Clark EA,?et al.?1996.?J. Biol. Chem.?271:14814.
Gene ID: 5594 View all products for this Gene ID
UniProt: View information about ERK1/2 Phospho Thr202 Tyr204 on UniProt.org

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