Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/T-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 147	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes.

Product Citations

Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed

RRID

AB_2571895 (BioLegend Cat. No. 369503) AB_2571896 (BioLegend Cat. No. 369504)

Antigen Details

Structure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution: Cytoplasm and the nucleus.
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.
Interaction: ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Phospho-Proteins, Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References: 1. Futran AS,?et al.?2013.?Curr. Biol.?23:R972.?
2. Mendoza MC,?et al.?2011.?Trends Biochem. Sci.?36:320.?
3. Chambard JC,?et al.?2007.?Biochim. Biophys. Acta.?1773:1299.?
4. Roux PP,?et al.?2004.?Microbiol. Mol. Biol. Rev.?68:320.?
5. Torii S,?et al.?2004.?J. Biochem.?136:557.?
6. Clark EA,?et al.?1996.?J. Biol. Chem.?271:14814.
Gene ID: 5594 View all products for this Gene ID
UniProt: View information about ERK1/2 Phospho Thr202 Tyr204 on UniProt.org

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