TotalSeq™-A1019 anti-mouse CD215 (IL-15R&alp-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 369	1

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD215 recombinant protein (33-205 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq?-A oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.
Additional Product Notes: TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CCTTGGCACCCGAGAATTCCA (PCR handle), and BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A (capture sequence). B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.
RRID: AB_2832537 (BioLegend Cat. No. 153507)

Antigen Details

Structure: IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology of IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains.
Distribution: It is found on a variety of subsets including dendritic cells, activated monocytes, memory CD8 T cells and NK/NKT cells.
Function: IL-15Rα is involved in T and B cell proliferation and development, preferentially for memory CD8⁺ T cell proliferation, and NK/NKT cell development
Ligand/Receptor: IL-15
Cell Type: Dendritic cells, Monocytes, NK cells, NKT cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1.?Giri JG,?et al. 1995.?EMBO J.?14:3654.
2.?Giron-Michel J,?et al. 2005.?Blood.?106:2302.
3.?Lorenzen I,?et al. 2005.?J. Biol. Chem.?281:6642.
4.?Sato N,?et al.?2007.?Proc. Natl. Acad. Sci. USA.?104:588.
5.?Anderson BG, Quinn LS. 2016.?Endocrinology.?157:1315
Gene ID: 16169 View all products for this Gene ID
UniProt: View information about CD215 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们