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Purified anti-FBXL5 Antibody
产品名称:
Purified anti-FBXL5 Antibody
产品类别:
抗体
产品编号:
672703
产品应用:
672703
[价格]
规格 价格 库存
25µg ¥ 101 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant mouse FBXL5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on PFA-fixed, Triton X-100-permeabilized NIH/3T3 cells. The clone produced nuclear staining that did not match the iron-responsiveness observed during western blotting. Because of this, we do not recommend using this clone for IF.

During product development testing, a protein with a slightly lower molecular weight than the target was observed during PD testing. This protein displayed a similar iron-responsiveness to the target band. It is unclear whether this band represents a minor FBXL5 degradation product or a truncated isoform.

This clone does not recognize human FBXL5. For detection of human FBXL5 by western blot, we recommend BioLegend clone 10F4H9D12 (Cat. No. 672604).

This clone has not been tested for IP.

RRID
AB_2832845 (BioLegend Cat. No. 672703) AB_2832846 (BioLegend Cat. No. 672704)

Antigen Details

Structure
FBXL5 is a 690 amino acid protein with a predicted molecular weight of 78.56 kD.
Distribution

Cytosol and nucleus/ubiquitously expressed

Function
Regulates iron homeostasis
Antigen References
  1. Johnson NB, et al. 2017. J. Biol. Chem. 292:15976.
  2. Vinas-Castells R, et al. 2014. Nucleic Acids Res. 42:1079.
  3. Moroishi T, et al. 2014. J. Biol. Chem. 289:16430.
  4. Ruiz JC, et al. 2013. J. Biol. Chem. 288:552.
  5. Chollangi S, et al. 2012. J. Biol. Chem. 287:23710.
  6. Moroishi T, et al. 2011. Cell Metab. 14:339.
  7. Salahudeen AA, et al. 2009 Science. 326:722.
  8. Vashisht AA, et al. 2009 Science. 326:718.
Gene ID
242960 View all products for this Gene ID
UniProt
View information about FBXL5 on UniProt.org

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