PE anti-IκB-α Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 165	1

Product Details

Verified Reactivity

Human, Hamster

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full length human IκB-α recombinant protein expressed in E. coli.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.0075 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.

Product Citations

Lu L, et al. 2022. Cancers (Basel). 14:. PubMed

RRID

AB_2783365 (BioLegend Cat. No. 662411) AB_2783366 (BioLegend Cat. No. 662412)

Antigen Details

Structure: Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution: Cytoplasm
Function: Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction: NFkB1, SUMO4, p53, ribosomal S6 kinase 1
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Tumor Suppressors
Antigen References: 1. Baeuerle PA. 1998. Cell 95:729.
2. Hoffmann A,?et al. 2002. Science 298:1241.
3. Ito CY,?et al. 1995. Genomics 29:490.
Gene ID: 4792 View all products for this Gene ID
UniProt: View information about IkappaB-alpha on UniProt.org

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