Purified anti-Oct4 (Oct3) Antibody
- 产品名称：
- Purified anti-Oct4 (Oct3) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 653701
- 产品应用：
- 653701
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human Oct4 recombinant protein (1-141aa).
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ChIP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 ?g/ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration of 2.5 ?g/ml is recommended. For ChIP applications, the suggested use of this reagent is 1:50 to 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- Claassen JN et al. 2019. Stem Cell Res. 1723:12:00 . PubMed
- Guo Y et al. 2018. Stem cell research. 29:139-142 . PubMed
- Zhu T, et al. 2021. Stem Cell Research. 55:102502. PubMed
- Fu Q, et al. 2021. Stem Cell Research. 54:102428. PubMed
- Wu S, et al. 2021. Stem Cell Research. 53:102330. PubMed
- Grzela DP, et al. 2020. Stem Cell Research. 72.65138889. PubMed
- Xu Z, et al. 2021. Stem Cell Research. 53:102359. PubMed
- McLenachan S et al. 2019. Stem Cell Res. 1723:17:00 . PubMed
- RRID
- AB_2561766 (BioLegend Cat. No. 653701) AB_2561767 (BioLegend Cat. No. 653702)
- Structure
- Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
- Distribution
-
Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
- Function
- Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
- Interaction
- SOX2, SOX8, SOX10, androgen receptor, and ETS2
- Biology Area
- Cell Biology, Immunology, Stem Cells, Transcription Factors
- Antigen References
-
1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271.
- Gene ID
- 5460 View all products for this Gene ID
- UniProt
- View information about Oct4 on UniProt.org
