Purified anti-Tata Binding Protein Antibody
- 产品名称：
- Purified anti-Tata Binding Protein Antibody
- 产品类别：
- 抗体
- 产品编号：
- 668305
- 产品应用：
- 668305
- Verified Reactivity
- Human, Mouse, Rat, Other mammalian species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: gel supershift.
?
Human TBP has 339 amino acid with predicted Mw 38 kD, while mouse TBP has 316 amino acid with predicted Mw 35 kD. Clone 1TBP18 recognizes epitope 1-20.
- Application References
(PubMed link indicates BioLegend citation) -
- Thompson NE, et al. 2004. Protein Expr. Purif. 36:186.
- Product Citations
-
- Wang N, et al. 2020. Am J Physiol Cell Physiol. 319:C922. PubMed
- Phelps CA, et al. 2019. Sci Rep. 9:7990. PubMed
- RRID
- AB_2721754 (BioLegend Cat. No. 668305) AB_2721755 (BioLegend Cat. No. 668306)
- Structure
- TBP is a 37 kD transcription factor that is involved in eukaryotic transcription initiation.
- Distribution
-
Nucleus
- Function
- General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
- Interaction
- Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3, SPIB, SNAPC1, SNAPC2 and SNAPC4, UTF1, BRF2, UBTF, GPBP1, HIV-1 Tat, herpes simplex virus 1 ICP4, TBP-TATA complex, LLPH, HSF1.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Hoffmann A, et al. 1990. Nature. 346:387.
- Peterson MG, et al. 1990. Science. 248:1625.
- Kwon H, et al. 1994. J Biol Chem. 269:30140.
- Comai L, et al. 1994. Science. 266:1966.
- Gene ID
- 8148 View all products for this Gene ID
- UniProt
- View information about Tata Binding Protein on UniProt.org
