Brilliant Violet 421™ anti-Nanog Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 435	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full length NANOG recombinant protein expressed in E. coli.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations

Sivakumar S, et al. 2022. Cell Rep. 38:110395. PubMed

RRID

AB_2566688 (BioLegend Cat. No. 674208)

Antigen Details

Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD and contains a homeobox domain that is responsible for DNA binding.
Interaction: Interacts with ZFP281, SMAD1, and SALL4.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Yin D, et al. 2012. Int. J. Mol. Med. 29:587.
2. Villasante A, et al. 2011. Cell Cycle 10:1488.
3. Theunissen TW, et al. 2011. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366:2222.
4. Pan G, et al. 2007. Cell Res. 17:42.
5. Kashyap V, et al. 2009. Stem Cells Dev. 18:1093.
6. Silva J, et al. 2009. Cell 138:722.
Gene ID: 79923 View all products for this Gene ID
UniProt: View information about Nanog on UniProt.org

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