Alexa Fluor® 647 anti-Nanog Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 382	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full length NANOG recombinant protein expressed in E. coli.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.?

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.?

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations

Petters J, et al. 2020. Stem Cell Research. 72.42222222. PubMed
V ?lkner C, et al. 2020. Stem Cell Research. 41:101606.. PubMed

RRID

AB_2650619 (BioLegend Cat. No. 674210)

Antigen Details

Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD and contains a homeobox domain that is responsible for DNA binding.
Interaction: Interacts with ZFP281, SMAD1, and SALL4.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Yin D, et al. 2012. Int. J. Mol. Med. 29:587.
2. Villasante A, et al. 2011. Cell Cycle 10:1488.
3. Theunissen TW, et al. 2011. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366:2222.
4. Pan G, et al. 2007. Cell Res. 17:42.
5. Kashyap V, et al. 2009. Stem Cells Dev. 18:1093.
6. Silva J, et al. 2009. Cell 138:722.
Gene ID: 79923 View all products for this Gene ID
UniProt: View information about Nanog on UniProt.org

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