PerCP/Cyanine5.5 anti-GATA3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 147	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Partial human GATA3 recombinant protein (1-258 aa).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Product Citations

Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
Liu Y, et al. 2021. Prostate. 81:29. PubMed
Matisz CE, et al. 2018. Am J Pathol. 188:2589. PubMed
Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed

RRID

AB_2563218 (BioLegend Cat. No. 653811) AB_2563219 (BioLegend Cat. No. 653812)

Antigen Details

Structure: 48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus.
Distribution: Nucleus
Function: GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T)GATA(A/G)-3' in the promoter region of genes, regulates T cell development, T_H2 cell differentiation, and luminal cell proliferation.
Interaction: Interacts with Smad3, FOG1, FOG2, and PU.1.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Yagi R, et al. 2011. Int. Immunol. 23:415.
2. Chou J, et al. 2010. J. Cell Physiol. 222:42.
3. Ho IC, et al. 2009. Nat. Rev. Immunol. 9:125.
4. Rothenberg EV, et al. 2008. Semin. Immunol. 20:236.
5. Kouros-Mehr H, et al. 2008. Curr. Opin. Cell Biol. 20:164.
6. Van Esch H, et al. 2000. Nature 406:419.
Gene ID: 2625 View all products for this Gene ID 14462 View all products for this Gene ID
UniProt: View information about GATA3 on UniProt.org

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