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Alexa Fluor® 647 anti-GATA3 Antibody
产品名称：
Alexa Fluor® 647 anti-GATA3 Antibody
产品类别：
抗体
产品编号：
653809
产品应用：
653809
[价格]
规格 价格 库存
25tests ￥ 147 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human GATA3 recombinant protein (1-258 aa).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  2. Whitehead GS, et al. 2022. JCI Insight. 7:. PubMed
  3. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  4. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  5. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  6. Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed
  7. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  8. Cheng J, et al. 2022. iScience. 25:103588. PubMed
  9. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
RRID
AB_2563216 (BioLegend Cat. No. 653809) AB_2563217 (BioLegend Cat. No. 653810)

Antigen Details

Structure
48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus.
Distribution

Nucleus

Function
GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T)GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation.
Interaction
Interacts with Smad3, FOG1, FOG2, and PU.1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Yagi R, et al. 2011. Int. Immunol. 23:415.
2. Chou J, et al. 2010. J. Cell Physiol. 222:42.
3. Ho IC, et al. 2009. Nat. Rev. Immunol. 9:125.
4. Rothenberg EV, et al. 2008. Semin. Immunol. 20:236.
5. Kouros-Mehr H, et al. 2008. Curr. Opin. Cell Biol. 20:164.
6. Van Esch H, et al. 2000. Nature 406:419.

Gene ID
2625 View all products for this Gene ID 14462 View all products for this Gene ID
UniProt
View information about GATA3 on UniProt.org

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