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首页 > 抗体蛋白 > 抗体
Purified anti-mouse Ki-67 Antibody
产品名称：
Purified anti-mouse Ki-67 Antibody
产品类别：
抗体
产品编号：
652401
产品应用：
652401
[价格]
规格 价格 库存
25µg ￥ 1246 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. For western blotting, suggested working dilution(s): Use 5 ?l per 5 ml antibody dilution buffer for each mini-gel (0.5??g/ml).?For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10.0 ?g/mL is suggested. Additionally, each lot of this antibody is quality control tested by our Ki-67 staining protocol below. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
  2. Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  2. Minamide K, et al. 2020. Sci Rep. 10:14639. PubMed
  3. Herring CA et al. 2017. Cell systems. 6(1):37-51 . PubMed
  4. Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
  5. von Klitzing E, et al. 2018. Sci Rep. 20:594. PubMed
  6. Medina-Reyes E, et al. 2015. Environ Res. 136:424. PubMed
  7. Yuan CL, et al. 2018. Exp Ther Med. 9:1420. PubMed
  8. Heimesaat MM, et al. 2018. Eur J Microbiol Immunol (Bp). 1864:1609. PubMed
  9. Zingg D et al. 2018. Cancer cell. 34(1):69-84 . PubMed
  10. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  11. Zappasodi R, et al. 2021. Nature. 591:652. PubMed
  12. Marable SS, et al. 2020. J Am Soc Nephrol. 2543:31. PubMed
  13. Brown EM, et al. 2019. Cell Host Microbe. 25:668. PubMed
  14. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  15. Matsumoto Y, et al. 2019. Sci Rep. 9:15244. PubMed
  16. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  17. Javary J, et al. 2021. Liver Int. 41:1423. PubMed
  18. Hallam D, et al. 2015. Exp Gerontol. 67: 72-79. PubMed
  19. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  20. Horiuchi S, et al. 2021. Sci Immunol. :eabm3131. PubMed
  21. Guillaumond F, et al. 2015. Proc Natl Acad Sci U S A. 112:2473. PubMed
  22. Wang C, et al. 2016. J Cell Biol. 212: 545-60. PubMed
  23. Hollinshead KER, et al. 2020. Cell Rep. 33:108231. PubMed
  24. Rore H, et al. 2021. Commun Biol. 4:802. PubMed
  25. Johnson SA, et al. 2021. Eur J Immunol. 51:3228. PubMed
  26. Villarreal-Ponce A, et al. 2020. Cell Rep. 33:108417. PubMed
  27. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  28. Men L, et al. 2018. Cell Physiol Biochem. 51:2185. PubMed
  29. Heimesaat MM, et al. 2019. Front Immunol. 10:49. PubMed
  30. Lkhagvadorj K, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 319:L742. PubMed
  31. Miller AJ, et al. 2020. Dev Cell. 117:53. PubMed
  32. Klose A, et al. 2018. JCSM Rapid Commun. 9:20222. PubMed
  33. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  34. Wang G, et al. 2019. Front Immunol. 1.380555556. PubMed
  35. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  36. Matsumori T, et al. 2020. Cancer Res. 80:5305. PubMed
  37. Madouri F, et al. 2017. J Allergy Clin Immunol. 139:1650. PubMed
  38. Maier B, et al. 2020. Nature. 580:257. PubMed
  39. Hennchen M, et al. 2015. J Neurosci. 35: 16531 - 16544. PubMed
  40. Lee J, et al. 2019. Yonsei Med J. 60:667. PubMed
  41. Chen M, et al. 2018. Am J Physiol Endocrinol Metab. 315:E72. PubMed
RRID
AB_11203533 (BioLegend Cat. No. 652401) AB_11204254 (BioLegend Cat. No. 652402)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

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