Brilliant Violet 605™ anti-mouse Ki-67 Antib-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 4088	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application References (PubMed link indicates BioLegend citation)

Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed

Product Citations

Best SA, et al. 2018. Cell Metab. 27:935. PubMed
Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
Rivadeneira DB, et al. 2020. Immunity. 51(3):548-560. PubMed
Rios-Doria J, et al. 2021. Front Oncol. 10:598477. PubMed
Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
Loukov D, et al. 2016. J Leukoc Biol. 100: 121 - 129. PubMed
K?lin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
Serr I, et al. 2016. Nat Commun. 7:10991. PubMed

RRID

AB_2562664 (BioLegend Cat. No. 652413)

Antigen Details

Structure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution: Nucleus and chromosome
Function: Required for cell cycle progression and proliferation
Interaction: Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 17345 View all products for this Gene ID
UniProt: View information about Ki-67 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们