Alexa Fluor® 700 anti-mouse Ki-67 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2058	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application References (PubMed link indicates BioLegend citation)

Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed

Product Citations

Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
Hill DL, et al. 2021. Elife. 10:. PubMed
Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
Pasciuto E, et al. 2020. Cell. 182:625. PubMed
Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
Xia L, et al. 2021. Cell Chemical Biology. 28(5):610-624.e5. PubMed
Song W, et al. 2022. Immunity. 55:290. PubMed
Jacobs L, et al. 2022. Cancer Gene Ther. 29:984. PubMed

RRID

AB_2564284 (BioLegend Cat. No. 652419) AB_2564285 (BioLegend Cat. No. 652420)

Antigen Details

Structure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution: Nucleus and chromosome
Function: Required for cell cycle progression and proliferation
Interaction: Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 17345 View all products for this Gene ID
UniProt: View information about Ki-67 on UniProt.org

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