Alexa Fluor® 647 anti-mouse Ki-67 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2058	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application References (PubMed link indicates BioLegend citation)

Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed

Product Citations

McCoy S 2014. Am J Physiol Lung Cell Mol Physiol. 307:618. PubMed
Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed
Benet Z, et al. 2021. Cell Reports. 34(6):108733. PubMed
Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed
Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
Chierico L, et al. 2017. PLoS One. 12(2):e0171815. PubMed
Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
Tedeschi A, et al. 2020. Nat Commun. 11:1746. PubMed
Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
Weis V, et al. 2016. Cell Mol Gastroenterol Hepatol. 2:131-157. PubMed
Shimizu H, et al. 2019. JCI Insight. 4:4. PubMed
Subbarayal B, et al. 2016. J Immunol. 197: 3464 - 3470. PubMed
Zhang X, et al. 2020. Glia. . PubMed

RRID

AB_2562138 (BioLegend Cat. No. 652407) AB_2562139 (BioLegend Cat. No. 652408)

Antigen Details

Structure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution: Nucleus and chromosome
Function: Required for cell cycle progression and proliferation
Interaction: Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 17345 View all products for this Gene ID
UniProt: View information about Ki-67 on UniProt.org

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