PerCP/Cyanine5.5 anti-ZAP70 Phospho (Tyr319)/Syk P-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1974	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Modified peptide.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

This antibody can also weakly react with Syk Phospho (Tyr352) on WB.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Product Citations

Gill PS, et al. 2022. Front Immunol. 13:872652. PubMed

RRID

AB_2687050 (BioLegend Cat. No. 683709) AB_2687051 (BioLegend Cat. No. 683710)

Antigen Details

Structure: 619 amino acids with a predicted molecular weight of 70 kD.
Distribution: Cytoplasm and cell membrane.
Function: Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-g1-dependent and Ras-dependent signaling cascades. Association with PLC-g1.
Interaction: NFAM1, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, and CD247/CD3Z.
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. 88:9166.
2. Arpaia E, et al. 1994. Cell 76:947.
3. Chan AC, et al. 1994. Science 264:1599.
4. Negishi I, et al. 1995. Nature 376:435.
5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279.
6. Klammt C, et al. 2015. Nat. Immunol. 16:961.
7. Weiss A. 1993. Cell 73:209.
8. Gong Q, et al. 2001. J. Exp. Med. 194:507.
Gene ID: 7535 View all products for this Gene ID
UniProt: View information about ZAP-70 Phospho Tyr319 Syk Phospho Tyr352 on UniProt.org

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