TotalSeq™-C1177 anti-human CLEC5A Antibody -上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 5166	1

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant protein (28 — 188 a.a.) expressed in 293E cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq?-C oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.
Additional Product Notes: TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.
RRID: AB_2892453 (BioLegend Cat. No. 371703)

Antigen Details

Structure: Approximately 21 kD C-type lectin domain-containing glyocoprotein.
Distribution: Expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes.
Function: Cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response.
Interaction: DAP12, TYROBP
Ligand/Receptor: Dengue Virus Envelope Proteins
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Antigen References: 1. Chen DY, et al. 2014. PLoS One e86105.
2. Wu MF, et al. 2013. Blood 121:95.
3. Yang YL, et al. 2012. J. Biomed. Biotechnol. 2012:398628.
4. Watson AA, et al. 2011. J. Biol. Chem. 286(27):24208.
Gene ID: 23601 View all products for this Gene ID
UniProt: View information about CLEC5A on UniProt.org

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