FITC anti-mouse CD64 (FcγRI) Antibody
- 产品名称：
- FITC anti-mouse CD64 (FcγRI) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 139316
- 产品应用：
- 139316
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BALB/c mouse FcγRI-human IgG Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
- Application References
(PubMed link indicates BioLegend citation) -
- Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
- Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
- Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed
- Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
- Khamissi FZ, et al. 2022. Sci Adv. 8:eabm5900. PubMed
- Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
- Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
- She L, et al. 2021. JCI Insight. 6:e143509. PubMed
- An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
- Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
- Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
- Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
- Liu J, et al. 2018. Am J Pathol. 188:2786. PubMed
- Liu SS, et al. 2021. Immunity. :. PubMed
- Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
- Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
- Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
- RRID
- AB_2566555 (BioLegend Cat. No. 139315) AB_2566556 (BioLegend Cat. No. 139316)
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
