Purified anti-NF-κB p65 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1736	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Partial human NF-κB p65 recombinant protein (451-551 aa)

Formulation

This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.

Concentration

0.5 mg/ml

Storage & Handling

Upon receipt, store undiluted between 2°C and 8°C.

Application

WB -?Quality tested
IP,?KO/KD-WB?- Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-1.0 ?g per ml. For immunoprecipitation, the suggested use of this reagent is 2-10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations

De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed

RRID

AB_2561612 (BioLegend Cat. No. 653001) AB_2561613 (BioLegend Cat. No. 653002)

Antigen Details

Structure: 65 kD protein containing a Rel homology domain (RHD), an activation domain, and a 9aaTAD domain.
Distribution: The inactivated NF-κB complex containing p65 subunit is bound to IκB and is localized to cytoplasm. Upon activation, I-κB is phosphorylated and degraded. The activated NF-κB complex is in turn translocated to the nucleus as a transcription factor.
Function: NF-κB is a homodimeric or heterodimeric complex formed by the Rel-like domain-containing proteins. The most abundant form is p65 (RelA) - p50 (NF-κB1) heterodimer complex. The NF-κB complex is a ubiquitously expressed transcription factor which is involved in various biological functions, such as cell growth, tumorigenesis, differentiation, apoptosis, inflammation, and immune responses.
Interaction: Interacts with NF-κB1 (p105/p50) or NF-κB2 (p100/p52) to form heterodimeric NF-κB complex. Interacts with HDAC1, HDAC3, and CBP. Interaction with MEN1 inhibits transactivation activity of NF-κB complex.
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Li Z, et al. 1997. Mol. Cell. Biol. 17:6184.
2. Saccani S, et al. 2004. J. Exp. Med. 200:107.
3. Nolan GP, et al. 1991. Cell 64:961.
4. Chen LF, et al. 2001. Science 293:1653.
5. Hansen SK, et al. 1994. Mol. Cell. Biol. 14:2593.
6. Chapman NR, et al. 2002. Biochem J. 366:459.
Gene ID: 4790 View all products for this Gene ID
UniProt: View information about NF-kappaB p65 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们