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APC anti-human CD31 Antibody
产品名称：
APC anti-human CD31 Antibody
产品类别：
抗体
产品编号：
303116
产品应用：
303116
[价格]
规格 价格 库存
100tests ￥ 3654 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8,?blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
  11. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  12. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed
  2. Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed
  3. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
  4. Cortesi EE, et al. 2021. Cells. 10:. PubMed
  5. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  6. Jung S, et al. 2015. J Rheumatol. 42:264. PubMed
  7. Wicki A, et al. 2012. Clin Cancer Res. 1.065277778. PubMed
  8. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
  9. Junghof J, et al. 2022. NPJ Regen Med. 7:14. PubMed
  10. Watanabe Y, et al. 2015. Mol Ther Nucleic Acids. 4: e253. PubMed
  11. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  12. Ye W, et al. 2022. Regen Ther. 21:477. PubMed
  13. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  14. Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed
  15. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
  16. Besnier M, et al. 2021. Antioxidants (Basel). 10:. PubMed
  17. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  18. de Sousa BM, et al. 2021. NPJ Regen Med. 6:80. PubMed
  19. Miki K, et al. 2021. Nat Commun. 12:3596. PubMed
  20. Takeda A, et al. 2020. Immunity. 51(3):561-572. PubMed
  21. Singh K, et al. 2017. Mol Ther. 25:2689. PubMed
  22. Chu PH, et al. 2020. Chem Res Toxicol. 751:33. PubMed
  23. Bharat A, et al. 2020. Science Translational Medicine. 12(574):. PubMed
  24. Takeda A, et al. 2020. STAR Protoc. 1:100021. PubMed
  25. Antonova LV, et al. 2019. Polymers (Basel). 11:1. PubMed
  26. Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed
  27. Wilson SB, et al. 2022. Genome Med. 14:19. PubMed
  28. Liu Z, et al. 2018. Cancer Med. 10.1002/cam4.1584. PubMed
RRID
AB_1877152 (BioLegend Cat. No. 303115) AB_1877151 (BioLegend Cat. No. 303116)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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