Alexa Fluor® 488 anti-SOX2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1568	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full length human SOX recombinant protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended.?It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations

Ceholski D, et al. 2017. Stem Cell Res. 10.1016/j.scr.2017.06.015. PubMed
Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
Chen G, et al. 2020. Stem Cell Research. 72.58402778. PubMed
Alexandrova S, et al. 2016. Development. 143: 24 - 34. PubMed
Coccini T, et al. 2022. Curr Protoc. 2:e423. PubMed

RRID

AB_2563956 (BioLegend Cat. No. 656109) AB_2563957 (BioLegend Cat. No. 656110)

Antigen Details

Structure: 317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution: Nucleus
Function: Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction: Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References: 1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228.
2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725.
3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772.
4. Jeong CH, et al. 2010. Stem Cells?28:2141.
5. Xiang R, et al. 2011. Br. J. Cancer 104:1410.
6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.
Gene ID: 6657 View all products for this Gene ID
UniProt: View information about SOX2 on UniProt.org

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