Brilliant Violet 650™ anti-mouse/human Ki-67
- 产品名称：
- Brilliant Violet 650™ anti-mouse/human Ki-67
- 产品类别：
- 抗体
- 产品编号：
- 151215
- 产品应用：
- 151215
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- RRID
- AB_2876504 (BioLegend Cat. No. 151215)
- Structure
- 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
- Distribution
-
Nucleus and chromosomes.
- Function
- Required for cell cycle progression and proliferation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
- Starborg M, et al. 1996. J. Cell. Sci. 109:143.
- Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
- Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
- Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
- Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
- Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID 17345 View all products for this Gene ID
- UniProt
- View information about Ki-67 on UniProt.org
