Brilliant Violet 421™ anti-mouse/human Ki-67
- 产品名称：
- Brilliant Violet 421™ anti-mouse/human Ki-67
- 产品类别：
- 抗体
- 产品编号：
- 151208
- 产品应用：
- 151208
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular? immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125??g per million cells in 100 ?l volume.?For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 μg/ml is suggested.?It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Product Citations
-
- Bieniasz–Krzywiec P, et al. 2020. Cell Metabolism. 30(5):917-936. PubMed
- Burns JC, et al. 2020. eLife. 9:00. PubMed
- Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
- Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
- Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
- RRID
- AB_2629748 (BioLegend Cat. No. 151208)
- Structure
- 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
- Distribution
-
Nucleus and chromosomes.
- Function
- Required for cell cycle progression and proliferation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
- Starborg M, et al. 1996. J. Cell. Sci. 109:143.
- Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
- Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
- Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
- Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
- Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID 17345 View all products for this Gene ID
- UniProt
- View information about Ki-67 on UniProt.org
