Alexa Fluor® 647 anti-mouse/human Ki-67 Antibo
- 产品名称：
- Alexa Fluor® 647 anti-mouse/human Ki-67 Antibo
- 产品类别：
- 抗体
- 产品编号：
- 151206
- 产品应用：
- 151206
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC?- Quality tested
ICC, IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by? intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is?≤0.125 ?g per million cells in 100 ?l volume.?For immunocytochemistry, a concentration range of?2.5 - 5.0 μg/ml is recommended.For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 1.25 - 5.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Product Citations
-
- Kobayashi T, et al. 2019. Cell. 176:982. PubMed
- Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
- Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
- RRID
- AB_2566801 (BioLegend Cat. No. 151206)
- Structure
- 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
- Distribution
-
Nucleus and chromosomes.
- Function
- Required for cell cycle progression and proliferation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
- Starborg M, et al. 1996. J. Cell. Sci. 109:143.
- Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
- Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
- Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
- Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
- Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID 17345 View all products for this Gene ID
- UniProt
- View information about Ki-67 on UniProt.org
