TotalSeq™-A Human Universal Cocktail, V1.0 -上海雅吉生物科技有限公司

规格	价格	库存
5tests	￥ 91056	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Formulation

Lyophilized from PBS containing stabilizers. Please reconstitute each tube with cell staining buffer as indicated in the application notes when ready to use. Make sure the cake is completely dissolved prior to using the cocktail for staining cells. The lyophilized cocktail material can vary in appearance and appear as either a lyophilized cake or a powder.

Preparation

This reagent is a combination of TotalSeq?-A oligo conjugated clones at optimal concentrations for single-cell sequencing analysis. See additional product notes for a list of clones.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The lyophilized antibody cocktail should be stored between 2°C and 8°C in powder form and in a sealed container with desiccant until ready to use. Once vial is opened, it is to be reconstituted immediately. After reconstitution cocktail should be used within 2 hours.

Application

PG - Quality tested

Recommended Usage

This panel has been optimized on human PBMCs. Using lysed whole blood or additional TotalSeq? antibodies may require further optimization. Please contact our technical service group for further information.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

Click here for protocol

Download the complete dataset for all targets

Additional Product Notes

TotalSeq??reagents are designed to profile protein expression at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CCTTGGCACCCGAGAATTCCA (PCR handle), and BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A (capture sequence). B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA[Barcode]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A..

?Download the excel file for a full list of clones and barcodes.
?Download the Cell Ranger antibody reference and UMI counting csv file.

RRID