PerCP/Cyanine5.5 anti-mouse/rat XCR1 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse/rat XCR1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 148207
- 产品应用：
- 148207
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
- Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
- Product Citations
-
- Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
- Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
- Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
- Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
- Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
- Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
- Fearon AE, et al. 2021. iScience. 24:103143. PubMed
- RRID
- AB_2564363 (BioLegend Cat. No. 148207) AB_2564364 (BioLegend Cat. No. 148208)
- Structure
- Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
- Distribution
-
Subset of CD8α+ conventional dendritic cells.
- Function
- Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
- Ligand/Receptor
- XCL1 and XCL2.
- Cell Type
- Dendritic cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.
- Gene ID
- 23832 View all products for this Gene ID 301086 View all products for this Gene ID
- UniProt
- View information about XCR1 on UniProt.org
