Brilliant Violet 421™ anti-mouse/rat XCR1 An
- 产品名称：
- Brilliant Violet 421™ anti-mouse/rat XCR1 An
- 产品类别：
- 抗体
- 产品编号：
- 148216
- 产品应用：
- 148216
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.
- Application References
(PubMed link indicates BioLegend citation) -
- Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
- Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
- Product Citations
-
- Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
- Liu J, et al. 2019. Immunity. 50:600. PubMed
- Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
- B?ttcher JP, et al. 2018. Cell. 172:1022. PubMed
- Flommersfeld S, et al. 2021. Immunity. :. PubMed
- Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
- Giampazolias E, et al. 2021. Cell. . PubMed
- Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
- Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
- Huang X, et al. 2021. Immunity. . PubMed
- RRID
- AB_2565230 (BioLegend Cat. No. 148216)
- Structure
- Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
- Distribution
-
Subset of CD8α+ conventional dendritic cells.
- Function
- Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
- Ligand/Receptor
- XCL1 and XCL2.
- Cell Type
- Dendritic cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.
- Gene ID
- 23832 View all products for this Gene ID 301086 View all products for this Gene ID
- UniProt
- View information about XCR1 on UniProt.org
