PE anti-mouse CD300LG (Nepmucin) Antibody
- 产品名称：
- PE anti-mouse CD300LG (Nepmucin) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 147104
- 产品应用：
- 147104
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Nepmucin-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications for the relevant formats include: immunoelectron microscopy of peripheral LN HEVs1.
- Application References
(PubMed link indicates BioLegend citation) -
- Umemoto E, et al. 2006. J. Exp. Med. 203:1603. (EM)
- Jin S, et al. 2008. FEBS Lett. 582:3018.
- Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.
- Umemoto E, et al. 2013. PLoS One 8:e83681.
- RRID
- AB_2562971 (BioLegend Cat. No. 147104)
- Structure
- Sialomucin, member of the CD300 antigen like family, 50-95 kD. 1 IgV like domain, 1 mucin like domain. Four isoforms produced by alternative splicing.
- Distribution
-
Expressed on high endothelial venules (HEVs) from lymph nodes but absent on HEVs from Peyer's patches, some myeloid cell lines.
- Function
- Mediator of lymphocyte adhesion and rolling on HEVs, possible role in transcytosis across the capillary endothelium.
- Ligand/Receptor
- L-selectin.
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Takatsu H, et al. 2006. Biochem. Biophys. Res. Commun. 348:183.
2. Umemoto E, et al. 2006. J. Exp. Med. 203:1603.
3. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.
- Gene ID
- 52685 View all products for this Gene ID
- UniProt
- View information about CD300LG on UniProt.org
