PerCP/Cyanine5.5 anti-mouse CD8b Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD8b Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126609
- 产品应用：
- 126609
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
- Product Citations
-
- Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
- Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
- Acker A, et al. 2014. PLoS One. 9:110015. PubMed
- Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
- Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
- LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
- Jin RM, et al. 2017. J Immunol. 198:352. PubMed
- Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
- Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
- Panea C, et al. 2021. Commun Biol. 4:913. PubMed
- de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
- LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
- Hassan AO, et al. 2020. Cell. 183:169. PubMed
- Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
- Gubin MM, et al. 2018. Cell. 175:1014. PubMed
- Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
- Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
- Bennion BG, et al. 2019. J Virol. 93. PubMed
- Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
- Sivaram N, et al. 2019. J Clin Invest. 130. PubMed
- Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
- Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
- RRID
- AB_961304 (BioLegend Cat. No. 126609) AB_2260149 (BioLegend Cat. No. 126610)
- Structure
- Ig superfamily, CD8β chain, 34 kD
- Distribution
-
Majority of thymocytes, cytotoxic T cell subsets
- Function
- Co-receptor of TCR
- Ligand/Receptor
- MHC class I
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
- Gene ID
- 12526 View all products for this Gene ID
- UniProt
- View information about CD8b on UniProt.org
