PE/Cyanine7 anti-mouse CD8b Antibody
- 产品名称：
- PE/Cyanine7 anti-mouse CD8b Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126615
- 产品应用：
- 126615
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
- Product Citations
-
- Sng XYX, et al. 2020. J Immunol. 204:3108. PubMed
- Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
- Lebel M?, et al. 2020. Nat Commun. 3.051388889. PubMed
- Holzki J, et al. 2015. J Virol. 89: 9886 - 9895. PubMed
- Ito F, et al. 2015. PLoS One. 10: 0143370. PubMed
- Kissiov DU, et al. 2022. Elife. 11:. PubMed
- Andreou T, et al. 2021. J Immunother Cancer. 9:. PubMed
- Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
- RRID
- AB_2562776 (BioLegend Cat. No. 126615) AB_2562777 (BioLegend Cat. No. 126616)
- Structure
- Ig superfamily, CD8β chain, 34 kD
- Distribution
-
Majority of thymocytes, cytotoxic T cell subsets
- Function
- Co-receptor of TCR
- Ligand/Receptor
- MHC class I
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
- Gene ID
- 12526 View all products for this Gene ID
- UniProt
- View information about CD8b on UniProt.org
