Alexa Fluor® 488 anti-mouse CD8b (Ly-3) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1736	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume.?For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.

Application References (PubMed link indicates BioLegend citation)

McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.

Product Citations

van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed

RRID

AB_2800618 (BioLegend Cat. No. 126627) AB_2800619 (BioLegend Cat. No. 126628)

Antigen Details

Structure: Ig superfamily, CD8β chain, 34 kD
Distribution: Majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand/Receptor: MHC class I
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
Gene ID: 12526 View all products for this Gene ID
UniProt: View information about CD8b on UniProt.org

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