Ultra-LEAF™ Purified anti-mouse CD54 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse NS-1 cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions^1,2,4, immunohistochemical staining³ of acetone-fixed frozen sections, immunoprecipitation⁴, and Western blotting (non-reducing). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Application References (PubMed link indicates BioLegend citation)

Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed

Product Citations

Sulimai N, et al. 2021. Biomolecules. 11:. PubMed
Sulimai N, et al. 2021. Int J Mol Sci. :22. PubMed

RRID

AB_2813980 (BioLegend Cat. No. 116131) AB_2813981 (BioLegend Cat. No. 116132) AB_2813982 (BioLegend Cat. No. 116133) AB_2813983 (BioLegend Cat. No. 116134) AB_2813984 (BioLegend Cat. No. 116135) AB_2813985 (BioLegend Cat. No. 116136)

Antigen Details

Structure: Ig superfamily, 90 kD
Distribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells
Function: Immune reaction, inflammation, adhesion
Ligand/Receptor: CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.
Gene ID: 15894 View all products for this Gene ID
UniProt: View information about CD54 on UniProt.org

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