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首页 > 抗体蛋白 > 抗体
Purified anti-mouse CD54 Antibody
产品名称：
Purified anti-mouse CD54 Antibody
产品类别：
抗体
产品编号：
116101
产品应用：
116101
[价格]
规格 价格 库存
50µg ￥ 756 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse NS-1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Application References

(PubMed link indicates BioLegend citation)
  1. Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
  2. Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
  3. Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
  4. Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
  5. Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
  6. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  7. Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
  8. Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
  9. El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
  10. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Yun S, et al. 2016. Nat Cell Biol. 18: 1043-1053. PubMed
  2. Tong CF, et al. 2018. Am J Physiol Cell Physiol. 315:C409. PubMed
  3. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  4. Greene C, et al. 2022. Nat Commun. 13:2003. PubMed
  5. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  6. Budatha M, et al. 2021. J Am Heart Assoc. 10:e021160. PubMed
  7. Greineder C, et al. 2013. PLoS One. 14:80110. PubMed
  8. Dertschnig S, et al. 2020. J Clin Invest. 130:1896. PubMed
  9. Mentrup T, et al. 2019. J Exp Med. 216:807. PubMed
  10. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  11. Wolf D, et al. 2018. Nat Commun. 9:525. PubMed
  12. Yun S, et al. 2019. J Clin Invest. 130:4863. PubMed
  13. Douglas G, et al. 2019. Cardiovasc Res. :. PubMed
  14. Liu J, et al. 2010. Proc Natl Acad Sci U S A. 107:16530. PubMed
  15. El-Assaad F, et al. 2013. Infect Immun . 81:3984. PubMed
  16. Lanitis E, et al. 2021. J Immunother Cancer. 9:. PubMed
  17. Wu C, et al. 2020. J Immunol. 204:212. PubMed
  18. Matsui Y 2007. Radiology . 243:386. PubMed
  19. Echeverria GV, et al. 2018. Nat Commun. 9:5079. PubMed
  20. Mehta V, et al. 2021. Sci Adv. 7:. PubMed
  21. Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed
  22. Clark VD, et al. 2018. Brain Res. 1699:158. PubMed
  23. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  24. Chacko A, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PubMed
  25. Wilson ZS, et al. 2020. Sci Rep. 10:2142. PubMed
  26. Seidler D, et al. 2011. J Immunol. 187:6108. PubMed
  27. Chen PY, et al. 2019. Nat Metab. 0.675. PubMed
  28. Saitoh S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01687-x. PubMed
  29. Tran NL, et al. 2022. Elife. 11:. PubMed
RRID
AB_313692 (BioLegend Cat. No. 116101) AB_313693 (BioLegend Cat. No. 116102)

Antigen Details

Structure
Ig superfamily, 90 kD
Distribution

Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Immune reaction, inflammation, adhesion
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Gene ID
15894 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

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