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PE anti-mouse CD54 Antibody
产品名称：
PE anti-mouse CD54 Antibody
产品类别：
抗体
产品编号：
116107
产品应用：
116107
[价格]
规格 价格 库存
50µg ￥ 1540 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse NS-1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Application References

(PubMed link indicates BioLegend citation)
  1. Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
  2. Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
  3. Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
  4. Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
  5. Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
  6. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  7. Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
  8. Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
  9. El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
  10. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Brenner JS, et al. 2018. Nat Commun. 9:20222. PubMed
  2. Cayre S, et al. 2020. Development. 147:00:00. PubMed
  3. Kitur K, et al. 2016. Cell Rep. 16:2219-2230. PubMed
  4. Chao TL, et al. 2020. Front Microbiol. 2.459722222. PubMed
  5. Dúcka M, et al. 2021. Neoplasia. 23:326. PubMed
  6. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  7. Kobori T, et al. 2018. Front Immunol. 9:334. PubMed
  8. Lachenmaier S, et al. 2011. J Neuroimmunol. 232:119. PubMed
  9. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  10. Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed
  11. Bresson L, et al. 2018. Development. 145. PubMed
  12. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  13. Moretti FA, et al. 2018. Elife. 7:e35816. PubMed
  14. Ise W, et al. 2018. Immunity. 48:702. PubMed
  15. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  16. Guilliams M, et al. 2022. Cell. 185:379. PubMed
  17. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  18. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  19. Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  20. Elgazar-Carmon V, et al. 2008. J Lipid Res. 49:1849. PubMed
  21. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  22. Korin B, et al. 2020. Sleep. :43. PubMed
  23. Corbet M, et al. 2021. PLoS Pathog. 17:e1010069. PubMed
  24. Cayre S, et al. 2020. Development. . PubMed
  25. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  26. Zhong J, et al. 2021. Commun Biol. 4:865. PubMed
RRID
AB_313698 (BioLegend Cat. No. 116107) AB_313699 (BioLegend Cat. No. 116108)

Antigen Details

Structure
Ig superfamily, 90 kD
Distribution

Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Immune reaction, inflammation, adhesion
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Gene ID
15894 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

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