APC anti-mouse CD54 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1260	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse NS-1 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions^1,2,4, immunohistochemical staining³ of acetone-fixed frozen sections, immunoprecipitation⁴, and Western blotting (non-reducing). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Application References (PubMed link indicates BioLegend citation)

Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed

Product Citations

Hutchins N, et al. 2013. Am J Pathol. 7:9440. PubMed
Regev O, et al. 2022. Front Immunol. 13:849701. PubMed
Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed
Rudraraju R, et al. 2012. Clin Vaccine Immunol. 19:757. PubMed
Fontinha D, et al. 2015. PLoS One. 10: 0142540. PubMed
Ross EC, et al. 2021. Cell Mol Life Sci. 78:5197. PubMed
Thomas AM, et al. 2020. Journal of Cellular Physiology. 235(6):5120-5129.. PubMed
Birkner K, et al. 2019. Front Neurol. 0.559027778. PubMed
Mor N et al. 2018. Cell stem cell. 23(3):412-425 . PubMed
Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
Ross EC, et al. 2022. Front Immunol. 13:950914. PubMed
Wang R, et al. 2021. JCSM Rapid Commun. 4:24. PubMed

RRID

AB_10613645 (BioLegend Cat. No. 116119) AB_10612936 (BioLegend Cat. No. 116120)

Antigen Details

Structure: Ig superfamily, 90 kD
Distribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells
Function: Immune reaction, inflammation, adhesion
Ligand/Receptor: CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.
Gene ID: 15894 View all products for this Gene ID
UniProt: View information about CD54 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们