Alexa Fluor® 647 anti-mouse CD54 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1414	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse NS-1 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions^1,2,4, immunohistochemical staining³ of acetone-fixed frozen sections, immunoprecipitation⁴, and Western blotting (non-reducing). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Application References (PubMed link indicates BioLegend citation)

Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed

Product Citations

Darbousset R, et al. 2012. Blood. 120:2133. PubMed
Zhou P, et al. 2011. Neurobiol Dis. 42:292. PubMed
De Niz M, et al. 2021. Cell Rep. 36:109741. PubMed
Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
Sadrai Z, et al. 2011. Invest Ophthalmol Vis Sci. 52:2525. PubMed
Palazón A, et al. 2011. Cancer Res. 71:801. PubMed
De Niz M, et al. 2022. STAR Protoc. 3:101450. PubMed

RRID

AB_493496 (BioLegend Cat. No. 116113) AB_493495 (BioLegend Cat. No. 116114)

Antigen Details

Structure: Ig superfamily, 90 kD
Distribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells
Function: Immune reaction, inflammation, adhesion
Ligand/Receptor: CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.
Gene ID: 15894 View all products for this Gene ID
UniProt: View information about CD54 on UniProt.org

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